Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation.
Identifieur interne : 000E32 ( Main/Exploration ); précédent : 000E31; suivant : 000E33Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation.
Auteurs : Akihito Nakanishi [Japon] ; Jun Gu Bae ; Kotaro Fukai ; Naoki Tokumoto ; Kouichi Kuroda ; Jun Ogawa ; Masato Nakatani ; Sakayu Shimizu ; Mitsuyoshi UedaSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2012.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Fermentation (MeSH), Laccase (génétique), Laccase (métabolisme), Oryza (microbiologie), Oryza (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Saccharomyces cerevisiae (enzymologie), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Trametes (enzymologie), Trametes (génétique), Éthanol (métabolisme).
- MESH :
- enzymologie : Saccharomyces cerevisiae, Trametes.
- génétique : Laccase, Protéines recombinantes, Saccharomyces cerevisiae, Trametes.
- microbiologie : Oryza.
- métabolisme : Laccase, Oryza, Protéines recombinantes, Saccharomyces cerevisiae, Éthanol.
- Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Fermentation.
English descriptors
- KwdEn :
- Cloning, Molecular (MeSH), Ethanol (metabolism), Fermentation (MeSH), Laccase (genetics), Laccase (metabolism), Molecular Sequence Data (MeSH), Oryza (metabolism), Oryza (microbiology), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Sequence Analysis, DNA (MeSH), Trametes (enzymology), Trametes (genetics).
- MESH :
- chemical , genetics : Laccase, Recombinant Proteins.
- chemical , metabolism : Ethanol, Laccase, Recombinant Proteins.
- enzymology : Saccharomyces cerevisiae, Trametes.
- genetics : Saccharomyces cerevisiae, Trametes.
- metabolism : Oryza, Saccharomyces cerevisiae.
- microbiology : Oryza.
- Cloning, Molecular, Fermentation, Molecular Sequence Data, Sequence Analysis, DNA.
Abstract
A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.
DOI: 10.1007/s00253-012-3876-8
PubMed: 22270238
Affiliations:
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Bae</name></author><author><name sortKey="Fukai, Kotaro" sort="Fukai, Kotaro" uniqKey="Fukai K" first="Kotaro" last="Fukai">Kotaro Fukai</name></author><author><name sortKey="Tokumoto, Naoki" sort="Tokumoto, Naoki" uniqKey="Tokumoto N" first="Naoki" last="Tokumoto">Naoki Tokumoto</name></author><author><name sortKey="Kuroda, Kouichi" sort="Kuroda, Kouichi" uniqKey="Kuroda K" first="Kouichi" last="Kuroda">Kouichi Kuroda</name></author><author><name sortKey="Ogawa, Jun" sort="Ogawa, Jun" uniqKey="Ogawa J" first="Jun" last="Ogawa">Jun Ogawa</name></author><author><name sortKey="Nakatani, Masato" sort="Nakatani, Masato" uniqKey="Nakatani M" first="Masato" last="Nakatani">Masato Nakatani</name></author><author><name sortKey="Shimizu, Sakayu" sort="Shimizu, Sakayu" uniqKey="Shimizu S" first="Sakayu" last="Shimizu">Sakayu Shimizu</name></author><author><name sortKey="Ueda, Mitsuyoshi" sort="Ueda, Mitsuyoshi" uniqKey="Ueda M" first="Mitsuyoshi" last="Ueda">Mitsuyoshi 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Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Bae, Jun Gu" sort="Bae, Jun Gu" uniqKey="Bae J" first="Jun Gu" last="Bae">Jun Gu Bae</name></author><author><name sortKey="Fukai, Kotaro" sort="Fukai, Kotaro" uniqKey="Fukai K" first="Kotaro" last="Fukai">Kotaro Fukai</name></author><author><name sortKey="Tokumoto, Naoki" sort="Tokumoto, Naoki" uniqKey="Tokumoto N" first="Naoki" last="Tokumoto">Naoki Tokumoto</name></author><author><name sortKey="Kuroda, Kouichi" sort="Kuroda, Kouichi" uniqKey="Kuroda K" first="Kouichi" last="Kuroda">Kouichi Kuroda</name></author><author><name sortKey="Ogawa, Jun" sort="Ogawa, Jun" uniqKey="Ogawa J" first="Jun" last="Ogawa">Jun Ogawa</name></author><author><name sortKey="Nakatani, Masato" sort="Nakatani, Masato" uniqKey="Nakatani M" first="Masato" last="Nakatani">Masato Nakatani</name></author><author><name sortKey="Shimizu, Sakayu" sort="Shimizu, Sakayu" uniqKey="Shimizu S" first="Sakayu" last="Shimizu">Sakayu Shimizu</name></author><author><name sortKey="Ueda, Mitsuyoshi" sort="Ueda, Mitsuyoshi" uniqKey="Ueda M" first="Mitsuyoshi" last="Ueda">Mitsuyoshi Ueda</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular (MeSH)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Laccase (genetics)</term><term>Laccase (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Oryza (metabolism)</term><term>Oryza (microbiology)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Saccharomyces cerevisiae (enzymology)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Trametes (enzymology)</term><term>Trametes (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Fermentation (MeSH)</term><term>Laccase (génétique)</term><term>Laccase (métabolisme)</term><term>Oryza (microbiologie)</term><term>Oryza (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Saccharomyces cerevisiae (enzymologie)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Trametes (enzymologie)</term><term>Trametes (génétique)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Laccase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term><term>Laccase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Saccharomyces cerevisiae</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Laccase</term><term>Protéines recombinantes</term><term>Saccharomyces cerevisiae</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Laccase</term><term>Oryza</term><term>Protéines recombinantes</term><term>Saccharomyces cerevisiae</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Fermentation</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Fermentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22270238</PMID><DateCompleted><Year>2012</Year><Month>08</Month><Day>15</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>94</Volume><Issue>4</Issue><PubDate><Year>2012</Year><Month>May</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation.</ArticleTitle><Pagination><MedlinePgn>939-48</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-012-3876-8</ELocationID><Abstract><AbstractText>A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakanishi</LastName><ForeName>Akihito</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bae</LastName><ForeName>Jun Gu</ForeName><Initials>JG</Initials></Author><Author ValidYN="Y"><LastName>Fukai</LastName><ForeName>Kotaro</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Tokumoto</LastName><ForeName>Naoki</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Kuroda</LastName><ForeName>Kouichi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ogawa</LastName><ForeName>Jun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Nakatani</LastName><ForeName>Masato</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Shimizu</LastName><ForeName>Sakayu</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ueda</LastName><ForeName>Mitsuyoshi</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>JF825479</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055454" MajorTopicYN="N">Trametes</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>10</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>12</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>12</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>8</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22270238</ArticleId><ArticleId IdType="doi">10.1007/s00253-012-3876-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Bae, Jun Gu" sort="Bae, Jun Gu" uniqKey="Bae J" first="Jun Gu" last="Bae">Jun Gu Bae</name><name sortKey="Fukai, Kotaro" sort="Fukai, Kotaro" uniqKey="Fukai K" first="Kotaro" last="Fukai">Kotaro Fukai</name><name sortKey="Kuroda, Kouichi" sort="Kuroda, Kouichi" uniqKey="Kuroda K" first="Kouichi" last="Kuroda">Kouichi Kuroda</name><name sortKey="Nakatani, Masato" sort="Nakatani, Masato" uniqKey="Nakatani M" first="Masato" last="Nakatani">Masato Nakatani</name><name sortKey="Ogawa, Jun" sort="Ogawa, Jun" uniqKey="Ogawa J" first="Jun" last="Ogawa">Jun Ogawa</name><name sortKey="Shimizu, Sakayu" sort="Shimizu, Sakayu" uniqKey="Shimizu S" first="Sakayu" last="Shimizu">Sakayu Shimizu</name><name sortKey="Tokumoto, Naoki" sort="Tokumoto, Naoki" uniqKey="Tokumoto N" first="Naoki" last="Tokumoto">Naoki Tokumoto</name><name sortKey="Ueda, Mitsuyoshi" sort="Ueda, Mitsuyoshi" uniqKey="Ueda M" first="Mitsuyoshi" last="Ueda">Mitsuyoshi Ueda</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Nakanishi, Akihito" sort="Nakanishi, Akihito" uniqKey="Nakanishi A" first="Akihito" last="Nakanishi">Akihito Nakanishi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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